Clonal dissemination of OXA-48-producing Enterobacter cloacaeisolates from companion animals in Algeria

Abstract

From 533 rectal swabs, 12 Enterobacteriaceae (2.3%), including 2 Escherichia coli, 2 Klebsiella pneumoniae and 8 E. cloacae, were recovered from selection plates. The 12 strains were resistant to amoxicillin/clavulanic acid, ticarcillin, piperacillin/tazobactam and ertapenem. All isolates were susceptible to aminoglycosides, imipenem and extended-spectrum cephalosporins. PCR and sequencing identified the blaOXA-48 gene in all isolates. qnrB1 was identified in all E. cloacae isolates. Plasmid analysis showed that the blaOXA-48 gene was localised on a 7-kb untypeable plasmid. RAPD analysis demonstrated the presence of the same profile pattern in the eight E. cloacae isolates. MLST analysis showed that the E. cloacae isolates belonged to ST527.