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| Élément Dublin Core | Valeur | Langue |
|---|---|---|
| dc.contributor.author | LAOUFI, Thiziri | - |
| dc.contributor.author | LADJ, Mouna | - |
| dc.date.accessioned | 2019-05-16T09:31:52Z | - |
| dc.date.available | 2019-05-16T09:31:52Z | - |
| dc.date.issued | 2017-07-03 | - |
| dc.identifier.citation | Université de Bouira | en_US |
| dc.identifier.uri | http://193.194.80.38:8080/jspui/handle/123456789/1451 | - |
| dc.description | Table of contents List of figures and tables List of abbreviations Introduction………………………………………………………………………………….......1 Literature review Chapter I: Artemisia herba-alba I-Botanical aspects of Artemisia herba alba (AHA)………………………………………...........3 I-1-Denominations…………………………………………………………………………......3 I-3-Morphology………………………………………………………………………………..3 I-4-Taxonomy………………………………………………………………………………….3 I-5-Distribution………………………………………………………………………………...4 I-6-Phytochemestry..……………………………………………………………………….......5 I-6-1-Sesquiterpene…………………………………………………………………………..5 I-6-2-Flavonoids……...……..……………………………………………………………….5 I-6-3-Phenolic compounds and waxes……………………………………………………….6 I-6-4-Essential oils…………………………………………………………………………...6 Chapter II: Peptic Ulcer Disease (stomach ulcers) II-1-Historical Perspective………………………………………………………………………..7 II-2-Definition……………………………………………………………………………………7 II-3-Signs and symptoms…………………………………………………………...…………….7 II-4-Anatomy……………………………………………………………………………...….......8 II-5-Pathophysiology…………………………………………………………………...……….10 II-6-Etiology……………………………………………………………………………….........10 II-6-1-Ethanol:………………………………………………………………………………..10 II-7-Epidemiology…………………………………………………………………………...….11 Chapter III : Skin inflammation III-1-Definition…………………………………………………………………………….........12 III-2-Etiologies ……………………………………………………………………….......12 III-3-Cells of inflammation…………………………………………………………………...13 III-4-Actors and course of inflammatory reaction……………………………………………13 III-4-1-Notions of acute inflammation and chronic inflammation……………………...….13 III-5-General procedure of the different stages of the inflammatory reaction………………......14 III-5-1-Vasculo-exudative reaction…………………………………………………………...14 III-5-1-1-Active congestion………………………………………………………………...14 III-5-1-2-Inflammatory Edema………………………………………………………….....15 Table of contents III-5-1-3-Leukocyte Diapedesis…………………………………………………………....15 III-6-Cell reaction…………………………………………………………………………….....16 III-6-1-Blood……………………………………………………………………………….....16 III-6-2-Local connective tissue…………………………………………………………….....16 III-7-Debridement……………………………………………………………………………….16 III-7-1-Internal Debridement…………………………………………………………………17 III-7-2-External Debridement………………………………………………………………...17 III-8-Repair and healing…………………………………………………………………………17 Experimental part Chapter I: Materials and methods I-Inflammatory assays…………………………………………………………………………...21 I-1-Plant material…………………………………………………………………………...21 I-1-1-Conservation…………………………………………………………………........21 I-2-Biological material………………………………………………………………………..21 I-3-Experimental design……………………………………………………………………...22 I-3-1-Groups of mice……………………………………………………………………….22 I-3-2-Preparation of the infused aqueous extract…………………………………………..22 I-4-Preparation of the ointment…………………………………………………………….23 I-5-Blood sampling and weight measurement……………………………………………......23 I-6-The biological parameters realized during the experimentation………………………….24 I-7-Methodes of determination of biochemical blood parameters……………………………24 I-7-1-Dosing of glucose…………………………………………………………………….24 I-7-2-Determination of total cholesterol……………………………………………………25 I-7-3-Determination of triglyceride………………………………………………………...25 I-7-4-Complete blood count (CBC) or Hemogram………………………………………...26 I-7-5-The creatinine dosage………………………………………………………………...26 I-7-6-The urea dosage………………………………………………………………………27 I-8-Realizations of blood smear………………………………………………………………27 II- Peptic ulcer disease (PUD)…………………………………………………………………..28 II-1- Test solution preparation……………………………………………………………......28 II-2- Experimental design…………………………………………………………………….28 Table of contents II-3-Dissection of mice……………………………………………………………………….29 II-3-1-Material………… ……………………………………………………………......29 II-3-2-The method of dissection…………………………………………………………...Erreur ! Signet non défini. II-4- Realization of histological sections………………………………………………………..31 II-4-1- Fixation………………………………………………………………...………….31 II-4-2- Inclusion…………………………………………………………………………..32 II-4-3- Sections……………………………………………………………..………………...32 II-4-4- The colorations…………………………………………………………..……......32 II-4-5- Mounting…………………………………………………………………...………32 II-5-Preparation technique in the laboratory of anatomopathology (stomach of mice)………...33 II-5-1- Fixation……………………………………………………………………………….33 II-5-2- Preparation before inclusion: Dehydration…………………………………………...33 II-5-3-Inclusion / coating………………………………………………..................................34 II-5-4- Histological section……………………………………………………………….......36 II-5-5-Attaching the cut to the blade…………………………………………………………36 II-5-6-Dewaxing……………………………………………………………………………...37 II-5-7-Dewaxing / coloring HE……………………………………………………………….38 II-5-8- Mounting……………………………………………………………………………...38 Chapter II: Results and discussion II-1-Results of blood smear study after 72 h of inflammation…………………………………..42 II-2- Results of the blood smear study after 21 days of treatment with the aqueous extract of Artemisia herba-alba…………………………………………………………………………42 II-3-Effect of the aqueous extract of Artemisia herba-alba on biochemical parameters……….43 II-3-1-White blood cells………………………………………………………………………43 II-3-2- Neutrophile……………………………………………………………………………44 II-3-3- Platelet………………………………………………………………………………...45 II-3-4-Blood glucose………………………………………………………………….............47 II-3-5-Cholesterol………………………………………………………………………….....48 II-3-6-Triglycerides…………………………………………………………………………..48 II-4-The tests performed to identify the presence of toxicity…………………………………...49 II-4-1-Urea……………………………………………………………………………………49 II-4-2-Creatinine ……………………………………………………………………………..50 Table of contents II-5- The influence of AHA administration on body weight change…………………………….51 II- 6-Peptic ulcer disease………………………………………………………………………..52 II-6-1- Histological sections……………………………………………………………........52 Conclusion………………………………………………………………………………………53 References Appendices | en_US |
| dc.description.abstract | Artemesia herba alba communément Appelée “Chih”, est une plante médicinal très répandue dans l’Afrique du nord largement utilise pour traiter plusieurs maladies. L’objectif de cette étude était d’évaluer l’effet anti inflammatoire et l’effet protecteur de l’estomac de l’extrait aqueux de l’armoise herbe blanche (1ml/kg pendant 21 jours) chez les souris blanches. Les résultats obtenu montrent en premier lieu que l’inflammation des souris induite par le stress lors de leur regroupement dans une seule cage a disparue après avoir nourri les souris par de l’extrait aqueux de l’armoise herbe blanche et l’application d’une pommade formée a partir de poudre de cette plante, mélangée avec de l’eau et appliquée sur les lésions de la peau. En deuxième, notre étude a montrée aussi que le gavage de l’extrait aqueux d’armoise blanche 60 minutes avant l’administration de l’éthanol a 50% aux souris a pu assurer une protection partial dela mucus gastrique. | en_US |
| dc.language.iso | fr | en_US |
| dc.publisher | Université de Bouira | en_US |
| dc.subject | armoise herbe blanche, extrait aqueux, inflammation, ulcère d’estomac, pommade | en_US |
| dc.title | Artemisia herba-alba anti-inflammatory activity and gastro-protective effects in mice | en_US |
| dc.type | Thesis | en_US |
| Collection(s) : | Mémoires Master | |
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