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Titre:	Intérêt des substances bioactives dans la protection des perturbations physiologiques liées au diabéte
Auteur(s):	CHEMLAL, Hanane
Date de publication:	2024
Editeur:	Université de BOUIRA جامعة البويرة
Référence bibliographique:	This thesis was conducted in order to evaluate the protective potential of some bioactive molecules, particularly vitamin C (Vit C) and vitamin E encapsulated in cyclodextrin (CD-Vit E) against the damage occurred in diabetes, especially on mature spermatozoa. The first part of this work consisted of monitoring the effect of diabetic plasma at different HbA1c levels on the RBCs and mature spermatozoa. This aimed to highlight the underlying mechanisms of diabetes-mediated cell damages in relation to HbA1c levels. The effect on RBCs was investigated by considering several biomarkers, known to be intimately related to diabetes as cell turbidity and MDA, regarding novel parameters, namely intracellular Hb and metHb production. The highest HbA1c levels induced several RBCs damages determined by the high level of hemolysis. The assessment of the intracellular molecules related this cytotoxic effect to the denaturation of Hb and simultaneously the overproduction of metHb. These were associated with the alteration of membrane cells illustrated by the microscopic analysis of the fastening by-products of Hb denaturation and oxidation to the cell cytoskeleton. Moreover, the measurement of MDA described the amplification of lipid peroxidation and the loss of membrane cell integrity. In parallel, the mature sperm cells exposed to diabetic plasma presented an important impairment in their quality. Indeed, the assessment of the kinematic parameters (VCL; VSL; and VAP) by the computer Sperm Class Analyzer® reported a significant decrease in sperm motility relatively to the rise of HbA1c. This directly correlated with the high peroxidation of the membrane’s lipid which was revealed by the rise of MDA values. Overall, these were mainly related to the exacerbation of OS in close relationship with the increase of HbA1c. However, this revealed the interest to investigate the protective effects of antioxidant supplementation in uncontrolled hyperglycemia. The second part of the study was based on the exploration of the cytoprotective potential of Vit C and CD-Vit E on sperm cells in diabetes conditions. Thus, we added a pre-treatment with Vit C and CD-Vit E at 0.25mg/ml and 0.1mg/ml, respectively to spermatozoa before incubation in diabetic plasma at high HbA1c. Indeed, their synergistic action allowed them to improve strongly the motility and the quality of mature spermatozoa. This was ensured by the attenuation of free radical damages on cells membrane showed by the decrease of lipid peroxidation. Therefore, these results suggest that the use of antioxidant combination supplements may add some protective potential in the future clinical management of poor control hyperglycemia.
URI/URL:	http://dspace.univ-bouira.dz:8080/jspui/handle/123456789/17610
Collection(s) :	Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre

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