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Title: | Enzymatic saccharification of solid residue of olive mill in a batch reactor |
Authors: | Lounici, Hakim |
Keywords: | Olive mill; Enzyme bioreactors; Saccharification; Batch processing; Optimisation; Product inhibition |
Issue Date: | Jan-2000 |
Publisher: | universite de bouira |
Citation: | Elsevier Science |
Abstract: | This paper describes the enzymatic hydrolysis of solid residue of olive mill (OMRS) in a batch reactor with the Trichoderma reesei enzyme. Before enzymatic saccharification, crude lignocellulosic material is submitted to alkaline pre-treatment with NaOH. Optimum conditions of the pre-treatment (temperature of T D 100 C and OMRS–NaOH concentration ratio of about R D 20) were determined. The optimum enzymatic conditions determined were as follows: pH of about 5, temperature of T D 50 C and enzyme to mass substrate mass ratio E=S D 0:1 g enzyme (g OMRS)−1. The maximum saccharification yield obtained at optimum experimental conditions was about 50%. The experimental results agree with Lineweaver Burk’s formula for low substrate concentrations. At substrate concentrations greater than 40 g dm−3, inhibitory effects were encountered. The kinetic constants obtained for the batch reactor were Km D 0:1 g dm−3 min−1 and V m D 800 g dm−3. © 2000 Elsevier Science S.A. All rights reserved. |
URI: | http://dspace.univ-bouira.dz:8080/jspui/handle/123456789/6596 |
Appears in Collections: | Articles |
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File | Description | Size | Format | |
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Enzymatic saccharification of solid residue of olive mill in a batch reactor.pdf | 303,93 kB | Adobe PDF | View/Open |
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