Please use this identifier to cite or link to this item: http://dspace.univ-bouira.dz:8080/jspui/handle/123456789/6596
Title: Enzymatic saccharification of solid residue of olive mill in a batch reactor
Authors: Lounici, Hakim
Keywords: Olive mill; Enzyme bioreactors; Saccharification; Batch processing; Optimisation; Product inhibition
Issue Date: Jan-2000
Publisher: universite de bouira
Citation: Elsevier Science
Abstract: This paper describes the enzymatic hydrolysis of solid residue of olive mill (OMRS) in a batch reactor with the Trichoderma reesei enzyme. Before enzymatic saccharification, crude lignocellulosic material is submitted to alkaline pre-treatment with NaOH. Optimum conditions of the pre-treatment (temperature of T D 100 C and OMRS–NaOH concentration ratio of about R D 20) were determined. The optimum enzymatic conditions determined were as follows: pH of about 5, temperature of T D 50 C and enzyme to mass substrate mass ratio E=S D 0:1 g enzyme (g OMRS)−1. The maximum saccharification yield obtained at optimum experimental conditions was about 50%. The experimental results agree with Lineweaver Burk’s formula for low substrate concentrations. At substrate concentrations greater than 40 g dm−3, inhibitory effects were encountered. The kinetic constants obtained for the batch reactor were Km D 0:1 g dm−3 min−1 and V m D 800 g dm−3. © 2000 Elsevier Science S.A. All rights reserved.
URI: http://dspace.univ-bouira.dz:8080/jspui/handle/123456789/6596
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